Overview

Methods in Stem Cell Engineering enables high school students to genetically engineer human stem cells using the cutting-edge technique,RNA interference (RNAi). Study the effects of silencing an oncogene in cancer stem cells,working hands-on in a biotech lab with professional industry scientists in this afterschool program.

INTRODUCTION.

Methods in Stem Cell Engineering is a four-session afterschool program exploring the science of gene modulation in stem cells. Participating students learn basic hands-on lab skills, using RNA interference (RNAi) to silence the expression of an oncogene in human cancer stem cells.

RNAi is a sophisticated technique that scientists use to inhibit the expression of specific genes in order to study their function. During Methods in Stem Cell Engineering, students learn bioinformatical approaches to designing an RNAi experiment and measure the effect of inhibiting the gene oct-4 in stem cells derived from testicular cancers. They also explore the real-world applications of RNAi technology, hearing from guest speakers and experts in the field of stem cell engineering.

CURRICULUM.

Intro to Stem Cell Systems focuses during each session on the following topics in stem cell engineering and gene silencing:

Session 1: Intro to cells, stem cells and RNAi gene silencing.

Session 2: Working with stem cells in the lab.

Session 3: RNAi mediated gene silencing techniques.

Session 4: Analysis and application of gene silencing.

 

KEY FEATURES.

– College-level lectures

– Hands-on lab classes

– Cutting edge techniques*

– Guest speakers

– Networking with professional science mentors

- Career education and free BioPaths career educatin webinar

*Intro to stem cell systems provides practical training in: BSL-2 lab safety procedures, aseptic technique, electroporation, RNAi mediated gene knockdown, antibody labeling and microscopy (phase contrast and fluorescent).

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